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p ampk α1 2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p ampk α1 2
    Source of primary antibodies and the dilutions for Western blotting or immunolocalization. NA, not applicable.
    P Ampk α1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 5243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p ampk α1 2/product/Cell Signaling Technology Inc
    Average 99 stars, based on 5243 article reviews
    p ampk α1 2 - by Bioz Stars, 2026-02
    99/100 stars

    Images

    1) Product Images from "Pifithrin-µ Induces Stress Granule Formation, Regulates Cell Survival, and Rewires Cellular Signaling"

    Article Title: Pifithrin-µ Induces Stress Granule Formation, Regulates Cell Survival, and Rewires Cellular Signaling

    Journal: Cells

    doi: 10.3390/cells13110885

    Source of primary antibodies and the dilutions for Western blotting or immunolocalization. NA, not applicable.
    Figure Legend Snippet: Source of primary antibodies and the dilutions for Western blotting or immunolocalization. NA, not applicable.

    Techniques Used: Western Blot

    PFT-µ diminishes the phosphorylation of AMPK on T172. Cells were incubated with vehicle (V) or PFT-µ for the hours [h] depicted. Crude extracts were evaluated for the phosphorylation of AMPK on T172 (p-AMPK). The same samples were also probed with antibodies against total AMPK (t-AMPK) and actin. The molecular mass of marker proteins in kD is indicated at the right margin. ECL signals were quantified for three independent experiments. Data were normalized to vehicle controls for each time point. Bars show average + SEM for each datapoint. Statistical evaluation was performed with one-way ANOVA combined with Bonferroni post hoc analysis. Changes were assessed relative to the vehicle control; ***, p < 0.001. AU, arbitrary units.
    Figure Legend Snippet: PFT-µ diminishes the phosphorylation of AMPK on T172. Cells were incubated with vehicle (V) or PFT-µ for the hours [h] depicted. Crude extracts were evaluated for the phosphorylation of AMPK on T172 (p-AMPK). The same samples were also probed with antibodies against total AMPK (t-AMPK) and actin. The molecular mass of marker proteins in kD is indicated at the right margin. ECL signals were quantified for three independent experiments. Data were normalized to vehicle controls for each time point. Bars show average + SEM for each datapoint. Statistical evaluation was performed with one-way ANOVA combined with Bonferroni post hoc analysis. Changes were assessed relative to the vehicle control; ***, p < 0.001. AU, arbitrary units.

    Techniques Used: Phospho-proteomics, Incubation, Marker, Control



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    (A) The relative mRNA levels of mechanistic target of rapamycin (mTOR). (B) A representative immunoblot of AMP-activated protein kinase <t>(AMPK),</t> threonine phosphorylation of AMP-activated protein kinase (P-AMPK), p70 S6 kinase (S6K), threonine phosphorylation of S6 kinase (P-S6K), and actin. Western blot densitometric analysis of (C) P-S6K/S6K. (D) P-AMPK/AMPK. Values are the mean ± SEM of three different blots. n = 5. ***p<0.001.
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    Image Search Results


    Source of primary antibodies and the dilutions for Western blotting or immunolocalization. NA, not applicable.

    Journal: Cells

    Article Title: Pifithrin-µ Induces Stress Granule Formation, Regulates Cell Survival, and Rewires Cellular Signaling

    doi: 10.3390/cells13110885

    Figure Lengend Snippet: Source of primary antibodies and the dilutions for Western blotting or immunolocalization. NA, not applicable.

    Article Snippet: p-AMPK-α1/2 , Cell Signaling , #2535 , 1:2000 , NA.

    Techniques: Western Blot

    PFT-µ diminishes the phosphorylation of AMPK on T172. Cells were incubated with vehicle (V) or PFT-µ for the hours [h] depicted. Crude extracts were evaluated for the phosphorylation of AMPK on T172 (p-AMPK). The same samples were also probed with antibodies against total AMPK (t-AMPK) and actin. The molecular mass of marker proteins in kD is indicated at the right margin. ECL signals were quantified for three independent experiments. Data were normalized to vehicle controls for each time point. Bars show average + SEM for each datapoint. Statistical evaluation was performed with one-way ANOVA combined with Bonferroni post hoc analysis. Changes were assessed relative to the vehicle control; ***, p < 0.001. AU, arbitrary units.

    Journal: Cells

    Article Title: Pifithrin-µ Induces Stress Granule Formation, Regulates Cell Survival, and Rewires Cellular Signaling

    doi: 10.3390/cells13110885

    Figure Lengend Snippet: PFT-µ diminishes the phosphorylation of AMPK on T172. Cells were incubated with vehicle (V) or PFT-µ for the hours [h] depicted. Crude extracts were evaluated for the phosphorylation of AMPK on T172 (p-AMPK). The same samples were also probed with antibodies against total AMPK (t-AMPK) and actin. The molecular mass of marker proteins in kD is indicated at the right margin. ECL signals were quantified for three independent experiments. Data were normalized to vehicle controls for each time point. Bars show average + SEM for each datapoint. Statistical evaluation was performed with one-way ANOVA combined with Bonferroni post hoc analysis. Changes were assessed relative to the vehicle control; ***, p < 0.001. AU, arbitrary units.

    Article Snippet: p-AMPK-α1/2 , Cell Signaling , #2535 , 1:2000 , NA.

    Techniques: Phospho-proteomics, Incubation, Marker, Control

    (A) The relative mRNA levels of mechanistic target of rapamycin (mTOR). (B) A representative immunoblot of AMP-activated protein kinase (AMPK), threonine phosphorylation of AMP-activated protein kinase (P-AMPK), p70 S6 kinase (S6K), threonine phosphorylation of S6 kinase (P-S6K), and actin. Western blot densitometric analysis of (C) P-S6K/S6K. (D) P-AMPK/AMPK. Values are the mean ± SEM of three different blots. n = 5. ***p<0.001.

    Journal: PLoS ONE

    Article Title: The Dietary Protein/Carbohydrate Ratio Differentially Modifies Lipogenesis and Protein Synthesis in the Mammary Gland, Liver and Adipose Tissue during Gestation and Lactation

    doi: 10.1371/journal.pone.0069338

    Figure Lengend Snippet: (A) The relative mRNA levels of mechanistic target of rapamycin (mTOR). (B) A representative immunoblot of AMP-activated protein kinase (AMPK), threonine phosphorylation of AMP-activated protein kinase (P-AMPK), p70 S6 kinase (S6K), threonine phosphorylation of S6 kinase (P-S6K), and actin. Western blot densitometric analysis of (C) P-S6K/S6K. (D) P-AMPK/AMPK. Values are the mean ± SEM of three different blots. n = 5. ***p<0.001.

    Article Snippet: The membranes were then incubated overnight at 4°C with the different primary antibodies as follows: FAS (Santa Cruz Biotechnology, sc-20140, 1∶2000), p70 S6 kinase α (Santa Cruz Biotechnology, sc-230, 1∶1000), p-p70 S6 kinase α (Santa Cruz Biotechnology, sc-11759, 1∶500), AMPK α1/2 (Santa Cruz Biotechnology, sc-25792, 1∶1000), P-AMPK α1/2 (Santa Cruz Biotechnology, sc-33524, 1∶500), and actin (Santa Cruz Biotechnology, sc-1615, 1∶1000), which were all diluted in 4 ml 1x TBS, 5% non-fat milk and 0.1% Tween 20.

    Techniques: Western Blot, Phospho-proteomics

    (A) The relative mRNA levels of sterol regulatory element binding protein 1 (SREBP1), fatty acid synthase (FAS), and pyruvate kinase (PK). (B) A representative immunoblot of AMP-activated protein kinase (AMPK), threonine phosphorylation of AMP-activated protein kinase (P-AMPK), p70 S6 kinase (S6K), threonine phosphorylation of S6 kinase (P-S6K), fatty acid synthase (FAS) and actin. (C) Western blot densitometric analysis of FAS/ACTIN. (D) A representative histology picture of the liver of rats fed 10/73, 20/63 or 30/53% DP/DCH at day 5 of lactation. Western blot densitometric analysis of (E) P-S6K/S6K, (F) P-AMPK/AMPK. (G) The relative mRNA levels of serine dehydratase (SDH). Values are the mean ± SEM of three different blots. n = 5. **p<0.01, ***p<0.001.

    Journal: PLoS ONE

    Article Title: The Dietary Protein/Carbohydrate Ratio Differentially Modifies Lipogenesis and Protein Synthesis in the Mammary Gland, Liver and Adipose Tissue during Gestation and Lactation

    doi: 10.1371/journal.pone.0069338

    Figure Lengend Snippet: (A) The relative mRNA levels of sterol regulatory element binding protein 1 (SREBP1), fatty acid synthase (FAS), and pyruvate kinase (PK). (B) A representative immunoblot of AMP-activated protein kinase (AMPK), threonine phosphorylation of AMP-activated protein kinase (P-AMPK), p70 S6 kinase (S6K), threonine phosphorylation of S6 kinase (P-S6K), fatty acid synthase (FAS) and actin. (C) Western blot densitometric analysis of FAS/ACTIN. (D) A representative histology picture of the liver of rats fed 10/73, 20/63 or 30/53% DP/DCH at day 5 of lactation. Western blot densitometric analysis of (E) P-S6K/S6K, (F) P-AMPK/AMPK. (G) The relative mRNA levels of serine dehydratase (SDH). Values are the mean ± SEM of three different blots. n = 5. **p<0.01, ***p<0.001.

    Article Snippet: The membranes were then incubated overnight at 4°C with the different primary antibodies as follows: FAS (Santa Cruz Biotechnology, sc-20140, 1∶2000), p70 S6 kinase α (Santa Cruz Biotechnology, sc-230, 1∶1000), p-p70 S6 kinase α (Santa Cruz Biotechnology, sc-11759, 1∶500), AMPK α1/2 (Santa Cruz Biotechnology, sc-25792, 1∶1000), P-AMPK α1/2 (Santa Cruz Biotechnology, sc-33524, 1∶500), and actin (Santa Cruz Biotechnology, sc-1615, 1∶1000), which were all diluted in 4 ml 1x TBS, 5% non-fat milk and 0.1% Tween 20.

    Techniques: Binding Assay, Western Blot, Phospho-proteomics

    (A) The relative mRNA levels of sterol regulatory element binding protein 1 (SREBP1), fatty acid synthase (FAS), and hormone sensitive lipase (HSL). (B) A representative immunoblot of AMP-activated protein kinase (AMPK), threonine phosphorylation of AMP-activated protein kinase (P-AMPK), p70 S6 kinase (S6K), threonine phosphorylation of S6 kinase (P-S6K), fatty acid synthase (FAS) and actin. Western blot densitometric analysis of (C) FAS/ACTIN, (D) P-S6K/S6K and (E) P-AMPK/AMPK. Values are the mean ± SEM of three different blots. n = 5. *p<0.05, **p<0.01 ***p<0.001.

    Journal: PLoS ONE

    Article Title: The Dietary Protein/Carbohydrate Ratio Differentially Modifies Lipogenesis and Protein Synthesis in the Mammary Gland, Liver and Adipose Tissue during Gestation and Lactation

    doi: 10.1371/journal.pone.0069338

    Figure Lengend Snippet: (A) The relative mRNA levels of sterol regulatory element binding protein 1 (SREBP1), fatty acid synthase (FAS), and hormone sensitive lipase (HSL). (B) A representative immunoblot of AMP-activated protein kinase (AMPK), threonine phosphorylation of AMP-activated protein kinase (P-AMPK), p70 S6 kinase (S6K), threonine phosphorylation of S6 kinase (P-S6K), fatty acid synthase (FAS) and actin. Western blot densitometric analysis of (C) FAS/ACTIN, (D) P-S6K/S6K and (E) P-AMPK/AMPK. Values are the mean ± SEM of three different blots. n = 5. *p<0.05, **p<0.01 ***p<0.001.

    Article Snippet: The membranes were then incubated overnight at 4°C with the different primary antibodies as follows: FAS (Santa Cruz Biotechnology, sc-20140, 1∶2000), p70 S6 kinase α (Santa Cruz Biotechnology, sc-230, 1∶1000), p-p70 S6 kinase α (Santa Cruz Biotechnology, sc-11759, 1∶500), AMPK α1/2 (Santa Cruz Biotechnology, sc-25792, 1∶1000), P-AMPK α1/2 (Santa Cruz Biotechnology, sc-33524, 1∶500), and actin (Santa Cruz Biotechnology, sc-1615, 1∶1000), which were all diluted in 4 ml 1x TBS, 5% non-fat milk and 0.1% Tween 20.

    Techniques: Binding Assay, Western Blot, Phospho-proteomics